| Name | Region |
|---|---|
| 01 Ventures | North Holland |
| 020drukwerk | North Holland |
| 040Hosting | |
| 1-2-appletree V.O.F. | |
| 100-downloads | North Holland |
| 1001 Online Games | North Holland |
| 100grams | North Holland |
| 10KB | |
| 10xStudio | |
| 123Domeinregistratie | North Brabant |
| 123inkt.nl | North Holland |
| 123Webshop | South Holland |
| 123websitenodig.nl | |
| 12design | North Holland |
| 12Return | South Holland |
| 15KM | North Holland |
| 1M2M | Utrecht |
| 1nspiring Holding B.V. | |
| 2-BBB Medicines B.V. | |
| 20Med Therapeutics | |
| 227 Learning | |
| 24i | North Holland |
| 24sessions | North Holland |
| 2develop | |
| 2E Interconnection B.V. | North Brabant |
| 2Eenheid Automatisering | South Holland |
| 2ICT | Gelderland |
| 30MHz | South Holland |
| 3D Hubs B.V. | |
| 3D Scan Solutions | |
| 3DLemon | North Holland |
| 3DPPM | North Brabant |
| 3dsoftware | |
| 3DUniversum | |
| 3po.nl | South Holland |
| 4 Your Games | |
| 40FINGERS.NET | Gelderland |
| 42 B.V. | South Holland |
| 42 Solutions | |
| 42workspace | |
| 4BI diensten | |
| 4dotnet | Drenthe |
| 4each B.V. | |
| 4Expand | North Holland |
| 4Impact | |
| 4Minds B.V. | Utrecht |
| 4net Group | Utrecht |
| 4Tops | Gelderland |
| 510.Ventures | |
| 5Groningen | Groningen |