| Internet Projekt, s.r.o. | Prague |
| Internethome, s.r.o. | Moravian-Silesian |
| INTERNEXT 2000, s.r.o. | Zlín |
| INTERSOFT - Automation, s.r.o. | |
| INTV spol. s r.o. | |
| Inven Capital | |
| Inventive Software, s.r.o. | Prague |
| Inveo.cz, s.r.o. | |
| Investování na Fundliftu | |
| Invia.cz, a.s. | |
| iolevel, s.r.o. | |
| iotaCSS | Prague |
| IPEX | Prague |
| iPhoneDoktor.cz | Prague |
| iPhoneServis.cz | Prague |
| Ipos-Soft, spol. s r.o. | |
| IQE Europe | |
| IR-webdesign | South Moravian |
| IT Signet, s.r.o. | Prague |
| IT-Help | |
| ITACA Business Incubator | Prague |
| itDesign | Plzeň |
| iTech21, s.r.o. | |
| Iterait, a.s. | |
| ITPA Technology, s.r.o. | Hradec Králové |
| ITPro CZ | Pardubice |
| J&T Ventures | Prague |
| Jan Bien | Prague |
| Jan Pivoňka | Prague |
| Jan Polzer | |
| JenaSoft | Prague |
| JetBrains, s.r.o. | Prague |
| JetSoft, s.r.o. | Prague |
| JettyVision, s.r.o. | Prague |
| Jetveo, s.r.o. | |
| Jetveo, s.r.o. | |
| Ježek software, s.r.o. | Liberec |
| JIC | South Moravian |
| Jihočeská univerzita v Českých Budějovicích | |
| JIMAZ, s.r.o. | Prague |
| Jimmy Technologies | |
| Jiří Ježek | Liberec |
| Jiri Stepanovsky | |
| JIRRA Software, s.r.o. | Pardubice |
| JK Education, s.r.o. | |
| JM-Net z.s. | Prague |
| Josef Stříbný | South Moravian |
| JPM Technologies, s.r.o. | |
| JRX automation, s.r.o. | |
| JS a partner, s.r.o. | |