| JSJT Automation, s.r.o. | |
| Juicymo, s.r.o. | Prague |
| JVM Computers, spol. s r.o. | Prague |
| JWA Prague, s.r.o. | |
| K-system.CZ, s.r.o. | |
| Kabelmánie, s.r.o. | South Moravian |
| Kancelář CATO, s.r.o. | |
| Kancelářské systémy, a.s. | |
| KARAT Software a.s. | Olomouc |
| Kardi AI Technologies, s.r.o. | |
| Karsa Technologies, s.r.o. | Hradec Králové |
| KAST, spol. s r.o. | Prague |
| KASTNER software, s.r.o. | Olomouc |
| Katedra informatiky FEI VŠB-TUO | Moravian-Silesian |
| Kaya | |
| KCT Data | |
| Keboola | |
| Keen Software House, s.r.o. | Prague |
| KELOC CS, s.r.o. | South Moravian |
| Kesh | |
| Kinalisoft, s.r.o. | |
| Kindred Group, s.r.o. | Prague |
| KMB systems, s.r.o. | Liberec |
| KMZ engineering, s.r.o. | |
| KnVnet services, s.r.o. | Central Bohemian |
| KOALA42 | Prague |
| KOMETA Plzeň, s.r.o. | |
| Komix | Prague |
| KompiTech, s.r.o. | |
| Kontent.ai | |
| Kontrolujeme, s.r.o. | |
| Koopeo Ventures | |
| KoPlac | |
| Korowatt, s.r.o. | |
| Kostax, s.r.o. | South Bohemian |
| Kostečka GROUP spol. s r.o. | |
| KP-SYS, spol. s r.o. | Pardubice |
| KROB software, s.r.o. | |
| Krofian CZ, s.r.o. | Liberec |
| Krup, s.r.o. | Prague |
| KSH-Data | Pardubice |
| KTK SOFTWARE, s.r.o. | Liberec |
| Kurent, s.r.o. | |
| Kvapem.cz | Prague |
| LabZone, s.r.o. | |
| Lakmoos AI, s.r.o. | |
| Lama plus | Moravian-Silesian |
| LAMIREL PCB Europe, s.r.o. | Hradec Králové |
| LangSoft, s.r.o. | |
| LazNet, s.r.o. | Prague |